Evaluation of Her2 Expression on Exosomes Secreted from Human Breast Cancer Cells by On-chip Immunoelectrophoresis toward Less-invasive Diagnosis
نویسندگان
چکیده
The expression of human epidermal growth factor receptor 2 (HER2) on the surface of individual exosomes secreted from human breast cancer cells was evaluated by on-chip microcapillary immunoelectrophoresis. A positive shift of the zeta potential was observed for exosomes secreted from HER2positive SK-BR-3 cells, whereas no difference was observed for exosomes secreted from HER2-negative MDA-MB-231 cells. These findings suggest that on-chip immunoelectrophoresis is a promising methodology for analyzing the surface HER2 protein on individual exosomes for future applications to lessinvasive HER2 testing.
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